Project B03 – Dynamics and function of posttranslational protein methylation and acetylation
The methylation and acetylation of lysine residues within proteins that regulate gene expression has been shown to play a key role in various diseases and the enzymes that catalyze these modifications have therefore gained considerable interest as potential drug targets. Surprisingly little is known about the proteins that are modified, the regulation of the enzymes, the dynamics of their turnover or their functional implications. Within this project we plan to use a variety of biochemical strategies to determine the complete lysine methylome of a human cell line. We will furthermore investigate the substrate specificity of a set of putative methyltransferases by applying a combination of different in vitro and in vivo assays. In addition, we will establish MALDI imaging as a new technology to investigate the distribution of inhibitors targeting such modifying enzymes and simultaneously the corresponding histone proteoform within tissues. Finally, we aim to use MALDI imaging as a new and antibody independent way to detect, quantify and characterize modified nucleic acids in situ.
Speaker: Prof. Dr. Thomas Carell
Ludwig-Maximilians-Universität München
Department of Chemistry Butenandtstr. 5 - 13
81377 Munich Germany
Phone: +49 (0)89 2180-77750
Fax: +49 (0)89 2180-77756
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Managing Director: Dr. Oliver Baron
Institute for Chemical Epigenetics Munich (ICEM)
Ludwig-Maximilians-Universität München
Butenandtstr. 5 - 13
81377 Munich Germany
Phone: +49 (0)89 2180-77661
Fax: +49 (0)89 2180-77651
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Secretary: Birgit Carell
Butenandtstr. 5 - 13 - Haus F 5.010
81377 Munich Germany
Phone: +49 (0)89 2180-77751
Fax: +49 (0)89 2180-77881
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Prof. Dr. Hendrik Zipse (Spokesman)
LMU Munich
Department of Chemistry
Butenandtstr. 5 - 13
House F, Room 3.084
81377 Munich, Germany
Phone: +49 89 2180 77737
Fax: +49 89 2180-77738
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Dr. Stefanie Kellner (Deputy Spokeswoman)
LMU Munich
Department of Chemistry
Butenandtstr. 5 - 13
House F, Room 1.054 und House B, Room 2.073
81377 Munich Germany
Phone: +49 89 2180 77737
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Dr. Martin Sumser (Coordinator)
LMU Munich
Department of Chemistry
Butenandtstr. 5 - 13
House F, Room 4.086
81377 Munich Germany
Phone: +49 89 2180 77765
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